Sarılop İncir (Ficus carica L.) Çeşidi Yaprak Segmentlerinden Somatik Embriyogenesis Oluşumu

نویسندگان

چکیده

Bu çalışmada, iyi kalitede sofralık ve kurutmalık bir incir çeşidi olan Sarılop çeşidinin yaprak segmentleri kullanılarak direkt indirekt somatik embriyogenesis yoluyla embriyo oluşumu amaçlanmıştır. Çalışmada eksplant olarak; 2011-2013 yıllarında Kasım Nisan aylarında alınan tepe tomurcuklarından Murashige-Skoog (MS) besin ortamında geliştirilen yapraklar kullanılmıştır. eksplantları, gelişimi için ilkbahar döneminde K-2 (2 mg/L 2,4-D (2,4-Dichlorophenoxyacetic asit) + 0.2 kinetin) ortam kombinasyonunda kültüre alınmıştır. % 66.66 oranında kallus elde edilmiştir; ancak edilememiştir. Direkt ise eksplantları TDZ (Thidiazuron) 2İP’in (N6-2-isopentenyladenine) farklı kombinasyonlarını içeren MS Eksplantlar kültüre; oluşumu, uzaması, kök ile şeklinde cevap vermiştir. En yüksek oranda oluşum oranı (% 42.76) en 20) DE-4 (MS 2 8 2İP) edilmiştir. Eksplant başına oluşan sayısı 0.83’tür. DE-2 4 83 gelişmesi de gözlenmiştir.

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ژورنال

عنوان ژورنال: Adnan menderes üniversitesi ziraat fakültesi dergisi

سال: 2022

ISSN: ['1304-7787']

DOI: https://doi.org/10.25308/aduziraat.1011855